Toxic waste disposal in Escherichia coli.
نویسندگان
چکیده
About 10% of the nalidixic acid-resistant (Nal(r)) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of acrAB was increased in each type of Nal(r) mutant. In the icdA and purB mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism.
منابع مشابه
Occurrence of genes associated with enterotoxigenic and enterohemorrhagic Escherichia coli in agricultural waste lagoons.
The prevalence among all Escherichia coli bacteria of the LTIIa toxin gene and STII toxin gene, both associated with enterotoxigenic E. coli, and of three genes (stxI, stxII, and eaeA) associated with enterohemorrhagic E. coli was determined in farm waste disposal systems seasonally for 1 year. Single- and nested-PCR results for the number of E. coli isolates carrying each toxin gene trait were...
متن کاملHigh-sensitivity hybridization assay for quantitation of residual E. coli DNA.
Impurity assays for recombinant protein therapeutics are essential to ensure batch-to-batch consistency and to meet the FDA's criteria for a well-characterized biopharmaceutical. For determination of residual host cell DNA, membrane hybridization assays utilizing radiolabeled DNA probes prepared from the host cell's genomic DNA have traditionally been used for products derivedfrom bacterial exp...
متن کاملEffect of Heavy Metal Uptake by E. coli and Bacillus sps
Over the past century, unrestricted mining, extensive industrialization, modern agricultural practices and faulty waste disposal methods have resulted in the release of unprecedented levels of toxic heavy metals like Cd, Hg, Ag, Sn, Pb, Cu, Co, Mn, Zn, etc into the environment. Many metals are essential for microbial growth in less concentration, yet are toxic in higher concentrations. Biosorpt...
متن کاملA Review on Use of Genetically Engineered Microorganisms for Bioremediation of Environmental Pollutants and Heavy Metals
Bioremediation refers to the use of organisms to remove environmental pollutants. Besides identifying different new plasmids capable of degrading environmental pollutants, many attempts have been taken by the genetic engineers to enhance the bioremediation potential of different microorganisms. Escherichia coli was engineered to convert chlorinated solvents such as trichloro ethylene and the hi...
متن کاملGrand Rounds: An Outbreak of Toxic Hepatitis among Industrial Waste Disposal Workers
CONTEXT Industrial waste (which is composed of various toxic chemicals), changes to the disposal process, and addition of chemicals should all be monitored and controlled carefully in the industrial waste industry to reduce the health hazard to workers. CASE PRESENTATION Five workers in an industrial waste plant developed acute toxic hepatitis, one of whom died after 3 months due to fulminant...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 184 13 شماره
صفحات -
تاریخ انتشار 2002